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continuous-flow chambers with polycarbonate filters  (AVESTIN Inc)
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AVESTIN Inc continuous-flow chambers with polycarbonate filters
ATP and glycerol 3-phosphate formation with continuous substrate feed. (A, B) Vesicles are retained by the <t>polycarbonate</t> filter (50 nm pore diameter). (A) Schematic of the experimental setup; 2.78 mg/mL of vesicles are added to the vesicle compartment and sampled over time. A flow of 50 mM KPi pH 7.0 is applied to the feed compartment. (B) LC-MS data normalized for dilution reveals that the total lipid composition is constant over time ( n = 2; error bars represent s.e.m.). (C, D) Metabolites equilibrate through the polycarbonate filter. (C) Schematic of the experimental setup. A metabolite gradient is imposed by applying 5 mM l -arginine/ l -ornithine/ l -citrulline in 50 mM KPi pH 7.0 to either of the chamber compartments. (D) HPLC data normalized for dilution reveal metabolite equilibration through the polycarbonate filter ( n = 4; error bars represent s.e.m.). Equilibration occurs at the same rate for both compartments. (E, F) ATP and glycerol 3-phosphate synthesis with a continuous l -arginine and glycerol feed. (E) Schematic of the experimental setup. The vesicles are applied to the vesicle compartment and the substrates are fed through the feed compartment. (F) Online ATP/ADP readout measured with PercevalHR ( n = 3; error bars represent s.e.m.).
Continuous Flow Chambers With Polycarbonate Filters, supplied by AVESTIN Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/continuous-flow chambers with polycarbonate filters/product/AVESTIN Inc
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continuous-flow chambers with polycarbonate filters - by Bioz Stars, 2026-06
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continuous flow-type oxidation chamber leco cs-044 carbon & sulfur 781-000 systems  (LECO Corporation)
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LECO Corporation continuous flow-type oxidation chamber leco cs-044 carbon & sulfur 781-000 systems
ATP and glycerol 3-phosphate formation with continuous substrate feed. (A, B) Vesicles are retained by the <t>polycarbonate</t> filter (50 nm pore diameter). (A) Schematic of the experimental setup; 2.78 mg/mL of vesicles are added to the vesicle compartment and sampled over time. A flow of 50 mM KPi pH 7.0 is applied to the feed compartment. (B) LC-MS data normalized for dilution reveals that the total lipid composition is constant over time ( n = 2; error bars represent s.e.m.). (C, D) Metabolites equilibrate through the polycarbonate filter. (C) Schematic of the experimental setup. A metabolite gradient is imposed by applying 5 mM l -arginine/ l -ornithine/ l -citrulline in 50 mM KPi pH 7.0 to either of the chamber compartments. (D) HPLC data normalized for dilution reveal metabolite equilibration through the polycarbonate filter ( n = 4; error bars represent s.e.m.). Equilibration occurs at the same rate for both compartments. (E, F) ATP and glycerol 3-phosphate synthesis with a continuous l -arginine and glycerol feed. (E) Schematic of the experimental setup. The vesicles are applied to the vesicle compartment and the substrates are fed through the feed compartment. (F) Online ATP/ADP readout measured with PercevalHR ( n = 3; error bars represent s.e.m.).
Continuous Flow Type Oxidation Chamber Leco Cs 044 Carbon & Sulfur 781 000 Systems, supplied by LECO Corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/continuous flow-type oxidation chamber leco cs-044 carbon & sulfur 781-000 systems/product/LECO Corporation
Average 90 stars, based on 1 article reviews
continuous flow-type oxidation chamber leco cs-044 carbon & sulfur 781-000 systems - by Bioz Stars, 2026-06
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continuous-flow streamwise thermal-gradient ccn chamber  (Mochida Pharmaceutical)
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Mochida Pharmaceutical continuous-flow streamwise thermal-gradient ccn chamber
ATP and glycerol 3-phosphate formation with continuous substrate feed. (A, B) Vesicles are retained by the <t>polycarbonate</t> filter (50 nm pore diameter). (A) Schematic of the experimental setup; 2.78 mg/mL of vesicles are added to the vesicle compartment and sampled over time. A flow of 50 mM KPi pH 7.0 is applied to the feed compartment. (B) LC-MS data normalized for dilution reveals that the total lipid composition is constant over time ( n = 2; error bars represent s.e.m.). (C, D) Metabolites equilibrate through the polycarbonate filter. (C) Schematic of the experimental setup. A metabolite gradient is imposed by applying 5 mM l -arginine/ l -ornithine/ l -citrulline in 50 mM KPi pH 7.0 to either of the chamber compartments. (D) HPLC data normalized for dilution reveal metabolite equilibration through the polycarbonate filter ( n = 4; error bars represent s.e.m.). Equilibration occurs at the same rate for both compartments. (E, F) ATP and glycerol 3-phosphate synthesis with a continuous l -arginine and glycerol feed. (E) Schematic of the experimental setup. The vesicles are applied to the vesicle compartment and the substrates are fed through the feed compartment. (F) Online ATP/ADP readout measured with PercevalHR ( n = 3; error bars represent s.e.m.).
Continuous Flow Streamwise Thermal Gradient Ccn Chamber, supplied by Mochida Pharmaceutical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/continuous-flow streamwise thermal-gradient ccn chamber/product/Mochida Pharmaceutical
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continuous-flow streamwise thermal-gradient ccn chamber - by Bioz Stars, 2026-06
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infant airlife single-limb, continuous-flow circuit, model ah132, containing airlife humidification chamber, model ah290 (recall # z-0343-2018)  (Vyaire Medical Inc)
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Vyaire Medical Inc infant airlife single-limb, continuous-flow circuit, model ah132, containing airlife humidification chamber, model ah290 (recall # z-0343-2018)
ATP and glycerol 3-phosphate formation with continuous substrate feed. (A, B) Vesicles are retained by the <t>polycarbonate</t> filter (50 nm pore diameter). (A) Schematic of the experimental setup; 2.78 mg/mL of vesicles are added to the vesicle compartment and sampled over time. A flow of 50 mM KPi pH 7.0 is applied to the feed compartment. (B) LC-MS data normalized for dilution reveals that the total lipid composition is constant over time ( n = 2; error bars represent s.e.m.). (C, D) Metabolites equilibrate through the polycarbonate filter. (C) Schematic of the experimental setup. A metabolite gradient is imposed by applying 5 mM l -arginine/ l -ornithine/ l -citrulline in 50 mM KPi pH 7.0 to either of the chamber compartments. (D) HPLC data normalized for dilution reveal metabolite equilibration through the polycarbonate filter ( n = 4; error bars represent s.e.m.). Equilibration occurs at the same rate for both compartments. (E, F) ATP and glycerol 3-phosphate synthesis with a continuous l -arginine and glycerol feed. (E) Schematic of the experimental setup. The vesicles are applied to the vesicle compartment and the substrates are fed through the feed compartment. (F) Online ATP/ADP readout measured with PercevalHR ( n = 3; error bars represent s.e.m.).
Infant Airlife Single Limb, Continuous Flow Circuit, Model Ah132, Containing Airlife Humidification Chamber, Model Ah290 (Recall # Z 0343 2018), supplied by Vyaire Medical Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/infant airlife single-limb, continuous-flow circuit, model ah132, containing airlife humidification chamber, model ah290 (recall # z-0343-2018)/product/Vyaire Medical Inc
Average 90 stars, based on 1 article reviews
infant airlife single-limb, continuous-flow circuit, model ah132, containing airlife humidification chamber, model ah290 (recall # z-0343-2018) - by Bioz Stars, 2026-06
90/100 stars
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infant airlife single-limb, continuous-flow circuit, model ah132, containing airlife humidification chamber, model ah290  (Vyaire Medical Inc)
90
Vyaire Medical Inc infant airlife single-limb, continuous-flow circuit, model ah132, containing airlife humidification chamber, model ah290
ATP and glycerol 3-phosphate formation with continuous substrate feed. (A, B) Vesicles are retained by the <t>polycarbonate</t> filter (50 nm pore diameter). (A) Schematic of the experimental setup; 2.78 mg/mL of vesicles are added to the vesicle compartment and sampled over time. A flow of 50 mM KPi pH 7.0 is applied to the feed compartment. (B) LC-MS data normalized for dilution reveals that the total lipid composition is constant over time ( n = 2; error bars represent s.e.m.). (C, D) Metabolites equilibrate through the polycarbonate filter. (C) Schematic of the experimental setup. A metabolite gradient is imposed by applying 5 mM l -arginine/ l -ornithine/ l -citrulline in 50 mM KPi pH 7.0 to either of the chamber compartments. (D) HPLC data normalized for dilution reveal metabolite equilibration through the polycarbonate filter ( n = 4; error bars represent s.e.m.). Equilibration occurs at the same rate for both compartments. (E, F) ATP and glycerol 3-phosphate synthesis with a continuous l -arginine and glycerol feed. (E) Schematic of the experimental setup. The vesicles are applied to the vesicle compartment and the substrates are fed through the feed compartment. (F) Online ATP/ADP readout measured with PercevalHR ( n = 3; error bars represent s.e.m.).
Infant Airlife Single Limb, Continuous Flow Circuit, Model Ah132, Containing Airlife Humidification Chamber, Model Ah290, supplied by Vyaire Medical Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/infant airlife single-limb, continuous-flow circuit, model ah132, containing airlife humidification chamber, model ah290/product/Vyaire Medical Inc
Average 90 stars, based on 1 article reviews
infant airlife single-limb, continuous-flow circuit, model ah132, containing airlife humidification chamber, model ah290 - by Bioz Stars, 2026-06
90/100 stars
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ATP and glycerol 3-phosphate formation with continuous substrate feed. (A, B) Vesicles are retained by the polycarbonate filter (50 nm pore diameter). (A) Schematic of the experimental setup; 2.78 mg/mL of vesicles are added to the vesicle compartment and sampled over time. A flow of 50 mM KPi pH 7.0 is applied to the feed compartment. (B) LC-MS data normalized for dilution reveals that the total lipid composition is constant over time ( n = 2; error bars represent s.e.m.). (C, D) Metabolites equilibrate through the polycarbonate filter. (C) Schematic of the experimental setup. A metabolite gradient is imposed by applying 5 mM l -arginine/ l -ornithine/ l -citrulline in 50 mM KPi pH 7.0 to either of the chamber compartments. (D) HPLC data normalized for dilution reveal metabolite equilibration through the polycarbonate filter ( n = 4; error bars represent s.e.m.). Equilibration occurs at the same rate for both compartments. (E, F) ATP and glycerol 3-phosphate synthesis with a continuous l -arginine and glycerol feed. (E) Schematic of the experimental setup. The vesicles are applied to the vesicle compartment and the substrates are fed through the feed compartment. (F) Online ATP/ADP readout measured with PercevalHR ( n = 3; error bars represent s.e.m.).

Journal: ACS Synthetic Biology

Article Title: ATP Recycling Fuels Sustainable Glycerol 3-Phosphate Formation in Synthetic Cells Fed by Dynamic Dialysis

doi: 10.1021/acssynbio.2c00075

Figure Lengend Snippet: ATP and glycerol 3-phosphate formation with continuous substrate feed. (A, B) Vesicles are retained by the polycarbonate filter (50 nm pore diameter). (A) Schematic of the experimental setup; 2.78 mg/mL of vesicles are added to the vesicle compartment and sampled over time. A flow of 50 mM KPi pH 7.0 is applied to the feed compartment. (B) LC-MS data normalized for dilution reveals that the total lipid composition is constant over time ( n = 2; error bars represent s.e.m.). (C, D) Metabolites equilibrate through the polycarbonate filter. (C) Schematic of the experimental setup. A metabolite gradient is imposed by applying 5 mM l -arginine/ l -ornithine/ l -citrulline in 50 mM KPi pH 7.0 to either of the chamber compartments. (D) HPLC data normalized for dilution reveal metabolite equilibration through the polycarbonate filter ( n = 4; error bars represent s.e.m.). Equilibration occurs at the same rate for both compartments. (E, F) ATP and glycerol 3-phosphate synthesis with a continuous l -arginine and glycerol feed. (E) Schematic of the experimental setup. The vesicles are applied to the vesicle compartment and the substrates are fed through the feed compartment. (F) Online ATP/ADP readout measured with PercevalHR ( n = 3; error bars represent s.e.m.).

Article Snippet: The continuous-flow chambers with 50 nm polycarbonate filters (Avestin) were preequilibrated with 50 mM KPi pH 7.0.

Techniques: Liquid Chromatography with Mass Spectroscopy